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KMID : 1204320100260020139
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Laboratory Animal Research
2010 Volume.26 No. 2 p.139 ~ p.144
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Fractionation of DNases Specific to Haemonchus contortus Intestine by Phenyl Sepharose Column
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Seo Min-Goo
Lee Young-Ju
Kwon Oh-Deog
Kwak Dong-Mi
Kim Seung-Joon
Jeong Kyu-Shik
Park Sang-Joon
Kim Tae-Hwan
Kwon Young-Sam
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Abstract
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Multiple DNases were identified from Haemonchus contortus intestine based on previous studies. The DNases detected at 34, 36 and 38.5 kDa had diverse characteristics. Some of them had characteristics similar to those of mammalians and others had unusual characteristics. This study was carried out to fractionate worm intestinal DNases from other proteins using phenyl Sepharose chromatographic methods. All DNases detected from Haemonchus contortus intestine were fractionated in the flowthrough of phenyl Sepharose, indicating the worm DNases are hydrophilic. The DNases were enriched five-fold in the flowthrough fraction while additional steps are required for isolation of the worm DNases. Thus, fractionation with phenyl Sepharose could be used as a good initial step to enrich and separate DNases from other proteins.
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KEYWORD
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DNases, Haemonchus contortus, intestine, chromatography, phenyl Sepharose
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