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KMID : 1204320100260020203
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Laboratory Animal Research
2010 Volume.26 No. 2 p.203 ~ p.209
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The Effect of Quercetin on the BKCa in Umbilical Cord Vein-derived Mesenchymal Stem Cells
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Park Kyoung-Sun
Kim Yang-Mi
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Abstract
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Recently, we demonstrated that a large conductance Ca2+-activated K+ channel (BKCa) in human umbilical cord vein-derived mesenchymal cells (hUC-MSCs). In this study, we studied effects of quercetin, the flavonoid used for the modulator of cell cycle and the treatment of anti-tumor, on BKca in hUC-MSCs using single channel and/or whole cell configuration. Single channel conductance was 277.8±36.2 pS in hUC-MSCs and the value is similar to that of typical BKCa channel reported in other cells. The single channel current was activated by increasing of intracellular Ca2+. Quercetin (30~100 μM) increased BKCa in whole cell patch configuration whereas EGCG was not affected on the channel. The activation effect of quercetin on BKCa channel was also observed in inside-out configuration. The quercetin-induced BKCa was increased a concentration-dependent manner with an IC50 value of 19.9 μM. Taken together, BKCa in hUCMSCs can be an important target for the action of quercetin and the channels are partly may contribute to maintaining homeostasis by regulation of K ion flux in hUC-MSCs.
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KEYWORD
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Human umbilical cord vein-derived mesenchymal cells, quercetin, BKCa
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