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KMID : 1204320100260030233
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Laboratory Animal Research
2010 Volume.26 No. 3 p.233 ~ p.239
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The Bone Grinding and Scaffold Grafting Techniques for Guide Bone Regeneration Induce the Stress on the Rat Brain
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Lee Yeo-Kyung
Kim Ji-Eun
Nam So-Hee
Choi Sun-Il
Lee Eon-Pil
Choi Hae-Wook
Kim Hong-Sung
Lee Jae-Ho
Jung Young-Jin
Kang Byeong-Cheol
Cho Jung-Sik
Hwang Dae-Youn
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Abstract
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Guided bone regeneration (GBR) is a technique that a barrier membrane is placed over the bone defect to prevent the cell growth from the connective tissue and epithelium. In this study, in order to determinewhether GBR technique could induce stress in rats, the standardized bone defect in rat calvaria was covered with apatitte membrane. Bone and brain tissues were collected from rats at 3 days, 2, 4, and 16 weeks post-operation, and then alteration of the new bone formation at the defects and stress-related factors were detected with histological examination and Western blot, respectively. From 4 to 16 weeks after the operation, the apatitte membrane was attached to the region of regenerated bone and encapsulated with a thick fibrous layer. Furthermore, the concentration of cortisol, a good indicator of stress, significantly increased 3 days post-operation. However, the increase at 3 days was returned to the basal level in 2 weeks. In Western blot analysis, the highest phosphorylation level of extracellular signalregulated kinase (ERK) was observed 3 day post-operation, while those of the c-jun N-terminal kinase (JNK) and p38 were detected 4 weeks post-operation. Taken together, the results suggest that GBR technique may induce the serious stress on the brain tissue via the induction of ERK phosphorylation during 2 weeks, and that the stress responses restored in 4 week via JNK and p38 signaling pathway.
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KEYWORD
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Guided bone regeneration, cortisol, mitogen-activated protein kinase (MAPK), stress, apatitte membrane
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