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KMID : 1204320110270010037
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Laboratory Animal Research
2011 Volume.27 No. 1 p.37 ~ p.40
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Determination of Oxyclozanide in Beef and Milk using High-Performance Liquid Chromatography System with UV Detector
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Jo Kyul
Cho Hee-Jung
Yi Hee
Cho Soo-Min
Park Jin-A
Kwon Chan-Hyeok
Park Hee-Ra
Kwon Ki-Sung
Shin Ho-Chul
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Abstract
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This study was developed and validated for the determination of oxyclozanide residue concentrations in beef and commercial milk, using high-performance liquid chromatography system. Oxyclozanide was successfully separated on a reverse phase column (Xbridge-C18, 4.6×250 ㎜, 5 ㎛) with a mobile phase composed of acetonitrile and 0.1% phosphoric acid (60:40, v/v%). This analytical procedure involved a deproteinization process using acetonitrile for beef and 2% formic acid in acetonitrile for commercial milk, dehydration by adding sodium sulfate to the liquid analytical sample, and a defatting process using n-hexane; after these steps, the extract was exposed to a stream of nitrogen dryness. The final extracted sample was dissolved in the mobile phase and filtered using a 0.45 ㎛ syringe filter. This method had good selectivity and recovery (70.70±7.90-110.79±14.95%) from the matrices. The LOQs ranged from 9.7 to 9.8 ㎍/㎏ for beef and commercial milk. The recoveries met the standards set by the CODEX guideline.
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KEYWORD
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Determination, oxyclozanide, beef, milk, HPLC
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