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KMID : 1204320110270040365
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Laboratory Animal Research
2011 Volume.27 No. 4 p.365 ~ p.368
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Antioxidative and aldose reductase-inhibitory effects of a fermentation filtrate of Rubus coreanus
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Kwon Sang-Chul
Kim Yun-Bae
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Abstract
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Antioxidative and aldose reductase (AR)-inhibitory effects of a fermentation filtrate of Rubus coreanus (FRC) were investigated using corneal/retinal homogenate and lens cytosol, respectively. Rat corneal/retinal homogenate was treated with 50 μM FeCl3 in the presence of FRC (3.2-100 μg/mL) for 30 min at 37℃, and thiobarbituric acid-reactive substances (TBARS) was quantified as a lipid peroxidation parameter. FRC markedly suppressed the TBARS production in a concentration-dependent manner, leading to 50% (IC50) and 100% (IC100) inhibitory concentrations of 20 and 95 μg/mL, respectively, which was similar to the effect of butylated hydroxyanisole. Activity of AR from rat lens was assayed in the presence of FRC (1-31.6 μg/mL) at 25℃ using glyceraldehyde as a substrate. FRC inhibited lens AR by 50% (IC50) and 90% (IC90) at approximately 2 and 31.6 μg/mL, respectively, comparable to the effect of quercetin. The results indicate that ERC could be a promising candidate for the improvement of eye injury and visual dysfunction of dry eye and diabetic patients.
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KEYWORD
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Rubus coreanus, antioxidative activity, aldose reductase inhibition
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