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KMID : 1204320140300020045
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Laboratory Animal Research
2014 Volume.30 No. 2 p.45 ~ p.53
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Proteomic analysis of domestic pig pancreas during development using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry
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Ahn Ji-Ye
Kim Il-Yong
Oh Sae-Jin
Hwang Hye-Sook
Yi Sun-Shin
Kim Yo-Na
Shin Jae-Hoon
Yoon Yeo-Sung
Seong Je-Kyung
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Abstract
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Pig pancreas may be a therapeutic resource for human diabetic patients. However, this potential is hindered by a lack of knowledge of the molecular events of pig pancreas development. In this study, the embryonic day 60, neonate and 6-month protein profiles of pig pancreas were ascertained at using two-dimensional gel electrophoresis and matrix assisted laser desorption/ionization-time of flight mass spectrometry. Twenty four proteins were differentially expressed during pig pancreas development. Among them, 12 spots increased and 7 spots decreased according to development. The expression of 5 protein were highest at birth. Expression of digestive enzymes including trypsin, pancreatic triacylglycerol lipase and pancreatic alpha-amylase was elevated in adults, whereas chymotrypsins were highly expressed in neonates. Proteins that were abundantly expressed during gestation were alpha-1-antitrypsin, alpha-fetoprotein and transferrins. Taken together, we found out that several proteins were significantly up- or down- regulated from pig pancreas based on developmental stage. This study will provide basis for understanding development of pig pancreas.
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KEYWORD
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Domestic pig, pancreas, development, 2-DE proteomics, MALDI-TOF
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