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KMID : 1204320150310030148
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Laboratory Animal Research
2015 Volume.31 No. 3 p.148 ~ p.152
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Extraction conditions of white rose petals for the inhibition of enzymes related to skin aging
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Choi Ehn-Kyoung
Guo Hai-Yu
Choi Jae-Kwon
Jang Su-Kil
Cha Ye-Seul
Choi Young-Jin
Seo Da-Woom
Lee Yoon-Bok
Joo Seong-Soo
Kim Yun-Bae
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Abstract
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In order to assess inhibitory potentials of white rose petal extracts (WRPE) on the activities of enzymes related to dermal aging according to the extraction conditions, three extraction methods were adopted. WRPE was prepared by extracting dried white rose (Rosa hybrida) petals with 50% ethanol (WRPE-EtOH), Pectinex® SMASH XXL enzyme (WRPE-enzyme) or high temperature-high pressure (WRPE-HTHP). In the inhibition of matrix metalloproteinase-1, although the enzyme activity was fully inhibited by all 3 extracts at 100 μg/mL in 60 min, partial inhibition (50-70%) was achieved only by WRPE-EtOH and WRPE-enzyme at 50 μg/mL. High concentrations (≥250 μg/mL) of all 3 extracts markedly inhibited the elastase activity. However, at low concentrations (15.6-125 μg/mL), only WRPE-EtOH inhibited the enzyme activity. Notably, WRPE-EtOH was superior to WRPE-enzyme and WRPE-HTHP in the inhibition of tyrosinase. WRPE-EtOH significantly inhibited the enzyme activity from 31.2 μM, reaching 80% inhibition at 125 μM. In addition to its strong antioxidative activity, the ethanol extract of white rose petals was confirmed to be effective in inhibiting skin aging-related enzymes. Therefore, it is suggested that WRPE-EtOH could be a good candidate for the improvement of skin aging such as wrinkle formation and pigmentation.
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KEYWORD
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Rosa hybrida, white rose petal extract, matrix metalloproteinase-1, elastase, tyrosinase
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