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KMID : 1204320160320040200
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Laboratory Animal Research
2016 Volume.32 No. 4 p.200 ~ p.207
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Diallyl disulfide attenuates acetaminophen-induced renal injury in rats
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Shin Jin-Young
Han Ji-Hee
Ko Je-Won
Park Sung-Hyeuk
Shin Na-Rae
Jung Tae-Yang
Kim Hyun-A
Kim Sung-Hwan
Shin In-Sik
Kim Jong-Choon
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Abstract
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This study investigated the protective effects of diallyl disulfide (DADS) against acetaminophen (AAP)-induced acute renal injury in male rats. We also investigated the effects of DADS on kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL), which are novel biomarkers of nephrotoxicity in renal tissues, in response to AAP treatment. The following four experimental groups were evaluated: (1) vehicle control, (2) AAP (1,000 mg/kg), (3) AAP&DADS, and (4) DADS (50 mg/kg/day). AAP treatment caused acute kidney injury evidenced by increased serum blood urea nitrogen (BUN) levels and histopathological alterations. Additionally, Western blot and immunohistochemistry analysis showed increased expression of KIM-1 and NGAL proteins in renal tissues of AAP-treated rats. In contrast, DADS pretreatment significantly attenuated the AAP-induced nephrotoxic effects, including serum BUN level and expression of KIM-1 and NGAL proteins. Histopathological studies confirmed the renoprotective effect of DADS. The results suggest that DADS prevents AAP-induced acute nephrotoxicity, and that KIM-1 and NGAL may be useful biomarkers for the detection and monitoring of acute kidney injury associated with AAP exposure.
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KEYWORD
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Acetaminophen, nephrotoxicity, KIM-1, NGAL, diallyl disulfide, protective effect
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