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KMID : 1204320170330020132
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Laboratory Animal Research
2017 Volume.33 No. 2 p.132 ~ p.139
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Comparison of humoral and cell-mediated immunity in three different C57BL/6N mouse substrains
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Kim Hye-Ryeong
Choi Jun-Young
Kim Kil-Soo
Jung Young-Suk
Cho Joon-Young
Hwang Dae-Youn
Song Hyun-Keun
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Abstract
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Adaptive immunity is a type of immune response mediated by T and B cells, and is important response for immune response amplification and memory. In this study, the adaptive immunologic properties of C57BL/6NKorl substrain were compared with those of two other C57BL/6N substrains. There were no significant differences between the C57BL/6NKorl and the two other C57BL/6N substrains in the histological structures of the thymus and spleen, which are immunologic organs containing T cell and B cells. In addition, flow cytometric analysis did not reveal any significant differences in the distribution of T and B cell populations of the three substrains. To evaluate cell-mediated immunity of T cells in the three different substrains, we treated isolated T cells from spleen with Con A. The T cells of C57BL/6NKorl showed Con A-dependent proliferation of T cells at lower cell number than those in T cells from the other two C57BL/6N substrains. B cell-mediated humoral immune responses were not significant different among the three substrains. Thus, the results of this study provide evidence that C57BL/6NKorl mice are similar to those two other C57BL/6N substrains in humoral immunity, but C57BL/6NKorl has stronger response in cell mediated immunity.
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KEYWORD
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C57BL/6N, C57BL/6NKorl, T cell, B cell, cell-mediated immunity, humoral immunity
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