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KMID : 1204320190350010016
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Laboratory Animal Research
2019 Volume.35 No. 1 p.16 ~ p.16
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Comparison of toxic responses to acetaminophen challenge in ICR mice originating from different sources
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Jeong Tae-Bin
Kim Joung-Hee
Kim Sou-Hyun
Lee Seung-Hyun
Son Seung-Won
Lim Yong
Cho Joon-Yong
Hwang Dae-Youn
Kim Kil-Soo
Kwak Jae-Hwan
Jung Young-Suk
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Abstract
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Acetaminophen (APAP) is the most common antipyretic analgesic worldwide. However, APAP overdose causes severe liver injury, especially centrilobular necrosis, in humans and experimental animals. At therapeutic dosage, APAP is mainly metabolized by sulfation and glucuronidation, and partly by cytochrome P450?mediated oxidation. However, APAP overdose results in production of excess reactive metabolite, N-acetyl-p-benzoquinone imine (NAPQI), by cytochromes P450; NAPQI overwhelms the level of glutathione (GSH), which could otherwise detoxify it. NAPQI binds covalently to proteins, leading to cell death. A number of studies aimed at the prevention and treatment of APAP-induced toxicity are underway. Rats are more resistant than mice to APAP hepatotoxicity, and thus mouse models are mainly used. In the present study, we compared the toxic responses induced by APAP overdose in the liver of ICR mice obtained from three different sources and evaluated the usability of the Korl:ICR stock established by the National Institute of Food and Drug Safety Evaluation in Korea. Administration of APAP (300?mg/kg) by intraperitoneal injection into male ICR mice enhanced CYP2E1 protein expression and depleted hepatic GSH level 2?h after treatment accompanied with significantly increased level of hepatic malondialdehyde, a product of lipid peroxidation. Regardless of the source of the mice, hepatotoxicity, as evidenced by activity of serum alanine aminotransferase, increased from 8?h and peaked at 24?h after APAP treatment. In summary, hepatotoxicity was induced after the onset of oxidative stress by overdose of APAP, and the response was the same over time among mice of different origins.
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KEYWORD
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Acetaminophen, Hepatotoxicity, GSH, ICR mouse
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