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KMID : 1204320190350010027
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Laboratory Animal Research
2019 Volume.35 No. 1 p.27 ~ p.27
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Antitumor effect of TW-37, a BH3 mimetic in human oral cancer
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Ahn Chi-Hyun
Lee Won-Woo
Jung Yun-Chan
Shin Ji-Ae
Hong Kyoung-Ok
Choi Su-Jung
Swarup Neeti
Kim Ji-Hoon
Jung Min-Jung
Cho Sung-Dae
Jin Bo-Hwan
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Abstract
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TW-37 is a small molecule B cell lymphoma-2 (Bcl-2) homology 3 mimetic with potential anticancer activities. However, the in vivo anti-cancer effect of TW-37 in human oral cancer has not been properly studied yet. Here, we attempted to confirm antitumor activity of TW37 in human oral cancer. TW-37 significantly inhibited cell proliferation and increased the number of dead cells in MC-3 and HSC-3 human oral cancer cell lines. TW-37 enhanced apoptosis of both cell lines evidenced by annexin V/propidium iodide double staining, sub-G1 population analysis and the detection of cleaved poly (ADP-ribose) polymerase and caspase-3. In addition, TW-37 markedly downregulated the expression of Bcl-2 protein, while not affecting Bcl-xL or myeloid cell leukemia-1. In vivo, TW-37 inhibited tumor growth in a nude mice xenograft model without any significant liver and kidney toxicities. Collectively, these data reveal that TW-37 may be a promising small molecule to inhibit human oral cancer.
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KEYWORD
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TW-37, Apoptosis, Bcl-2, Oral cancer
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