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KMID : 1204320190350010032
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Laboratory Animal Research
2019 Volume.35 No. 1 p.32 ~ p.32
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Four amino acids as serum biomarkers for anti-asthma effects in the ovalbumin-induced asthma mouse model treated with extract of Asparagus cochinchinensis
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Choi Jun-Young
Kim So-Hyun
Kim Ji-Eun
Park Ji-Won
Kang Mi-Ju
Choi Hyeon-Jun
Bae Su-Ji
Lee Jae-Ho
Jung Young-Suk
Hwang Dae-Youn
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Abstract
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The butanol extract of Asparagus cochinchinensis roots fermented with Weissella cibaria (BAW) effectively prevents inflammation and remodeling of airway in the ovalbumin (OVA)-induced asthma model. To characterize biomarkers that can predict the anti-asthmatic effects induced by BAW treatment, we measured the alteration of endogenous metabolites in the serum of OVA-induced asthma mice after administration of low concentration BAW (BAWLo, 250?mg/kg) and high concentration BAW (BAWHi, 500?mg/kg) using 1H nuclear magnetic resonance (1H-NMR) spectral data. The number of immune cells and serum concentration of IgE as well as thickness of the respiratory epithelium and infiltration of inflammatory cells in the airway significantly recovered in the OVA+BAW treated group as compared to the OVA+Vehicle treated group. In the metabolic profile analysis, the pattern recognition showed completely separate clustering of serum analysis parameters between the OVA+Vehicle and OVA+BAW treated groups. Of the total endogenous metabolites, 19 metabolites were upregulated or downregulated in the OVA+Vehicle treated group as compared to the Control treated group. However, only 4 amino acids (alanine, glycine, methionine and tryptophan) were significantly recovered after BAWLo and BAWHi treatment. This study provides the first results pertaining to metabolic changes in the asthma model mice treated with OVA+BAW. Additionally, these findings show that 4 metabolites can be used as one of biomarkers to predict the anti-asthmatic effects.
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KEYWORD
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Asparagus cochinchinensis, Fermentation, Weissella cibaria, Asthma, Therapeutic effects, Metabolomics, Amino acid
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