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KMID : 1204320220380010032
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Laboratory Animal Research
2022 Volume.38 No. 1 p.32 ~ p.32
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Key factors to establish the ovalbumin-induced atopic dermatitis minipig model: age and body weight
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Kim Young-Kyu
Lee Ju-Kyung
Kim Hyeon-Young
Kim Sung-Hwan
Hwang Jeong-Ho
Suh Han-Na
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Abstract
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Background: Given its similar structure and immune response to the human skin, porcine is a good model for dermal studies. Here, we sensitized ovalbumin (Ova) on minipig back skin for 2?4 weeks to induce chronic atopic dermatitis (AD).
Results: Gross observation, serum cytokine level, epidermal thickness, and epidermal integrity did not change after 4 weeks of Ova induction compared with the control, indicating AD modeling failure. Only the neutrophils in the blood and macrophages in bronchoalveolar lavage fluid changed slightly until 3 or 2 weeks after Ova sensitization, respectively. The successful and failed Ova-induced AD minipig models only differ in age and body weight of the minipigs. The minipigs, 12 months old with a 30-kg median weight, had a two-fold thicker dermis than minipigs 8?10 months old, with an 18.97-kg median weight, resulting in impaired Ova permeability and immune response.
Conclusion: Age and body weight are key factors that should be considered when developing an Ova-induced AD minipig model.
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KEYWORD
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Yucatan minipig, Atopic dermatitis, Ovalbumin, Age/body weight, Dermal thickness
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