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KMID : 1204720190120020340
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International Journal of Stem Cells
2019 Volume.12 No. 2 p.340 ~ p.346
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Hybrid Nanofiber Scaffold-Based Direct Conversion of Neural Precursor Cells/Dopamine Neurons
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Lim Mi-Sun
Ko Seung-Hwan
Kim Min-Sung
Lee Byung-Jun
Jung Ho-Sup
Kim Kee-Sung
Park Chang-Hwan
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Abstract
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The concept of cellular reprogramming was developed to generate induced neural precursor cells (iNPCs)/dopaminergic (iDA) neurons using diverse approaches. Here, we investigated the effects of various nanoscale scaffolds (fiber, dot, and line) on iNPC/iDA differentiation by direct reprogramming. The generation and maturation of iDA neurons (microtubule-associated protein 2-positive and tyrosine hydroxylase-positive) and iNPCs (NESTIN-positive and SOX2-positive) increased on fiber and dot scaffolds as compared to that of the flat (control) scaffold. This study demonstrates that nanotopographical environments are suitable for direct differentiation methods and may improve the differentiation efficiency.
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KEYWORD
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Hybrid nanofiber, Direct conversion, Neural precursor, iNPC
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