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KMID : 1204720190120020360
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International Journal of Stem Cells
2019 Volume.12 No. 2 p.360 ~ p.366
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MBP-FGF2-Immobilized Matrix Maintains Self-Renewal and Myogenic Differentiation Potential of Skeletal Muscle Stem Cells
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Sah Jay Prakash
Hao Nguyen Thi Thu
Kim Yun-Hye
Eigler Tamar
Tzahor Eldad
Kim Sang-Heon
Hwang Yong-Sung
Yoon Jeong-Kyo
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Abstract
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The robust capacity of skeletal muscle stem cells (SkMSCs, or satellite cells) to regenerate into new muscles in vivo has offered promising therapeutic options for the treatment of degenerative muscle diseases. However, the practical use of SkMSCs to treat muscle diseases is limited, owing to their inability to expand in vitro under defined cultivation conditions without loss of engraftment efficiency. To develop an optimal cultivation condition for SkMSCs, we investigated the behavior of SkMSCs on synthetic maltose-binding protein (MBP)-fibroblast growth factor 2 (FGF2)-immobilized matrix in vitro. We found that the chemically well-defined, xeno-free MBP-FGF2-immobilized matrix effectively supports SkMSC growth without reducing their differentiation potential in vitro. Our data highlights the possible application of the MBP-FGF2 matrix for SkMSC expansion in vitro.
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KEYWORD
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Skeletal muscle stem cell, Satellite cell, MBP-FGF2, Self-renewal, Myogenic differentiation
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