|
KMID : 1204720200130020202
|
|
International Journal of Stem Cells
2020 Volume.13 No. 2 p.202 ~ p.211
|
|
|
Overexpression of p21 Has Inhibitory Effect on Human Hematopoiesis by Blocking Generation of CD43＋ Cells via Cell-Cycle Regulation
|
|
Zeng Jiahui
Zhang Huifang
Liu Yuanling
Sun Wencui
Yi Danying
Zhu Lijiao
Zhang Yonggang
Pan Xu
Chen Yijing
Zhou Ya
Bian Guohui
Lai Mowen
Zhou Qiongxiu
Liu Jiaxin
Chen Bo
Ma Feng
|
|
|
Abstract
|
|
|
Background and Objectives: p21, an important member of the Cip/Kip family, is involved in inhibitory effects of RUNX1b overexpression during the early stage of human hematopoiesis.
Methods and Results: We established a human embryonic stem cell (hESC) line with inducible expression of p21 (p21/hESCs). Overexpression of p21 did not influence either mesoderm induction or emergence of CD34＋ cells, but it significantly decreased the production of CD43＋ cells and changed the expression profile of hematopoiesis-related factors, leading to the negative effects of p21 on hematopoiesis.
Conclusions: In RUNX1b/hESC co-cultures when RUNX1b was induced from D0, perturbation of the cell cycle caused by upregulation of p21 probably prevented the appearance of CD43＋ cells, but not CD34＋ cells. The mechanisms via which CD34＋ cells are blocked by RUNX1b overexpression remain to be elucidated.
|
|
|
KEYWORD
|
|
|
p21, RUNX1, CD43, Hematopoiesis, hPSC, AGM-S3, Inducible expression, PiggyBac, Cell cycle
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|