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KMID : 1204720200130020237
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International Journal of Stem Cells
2020 Volume.13 No. 2 p.237 ~ p.245
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SOCS1 Regulates the Immunomodulatory Roles of MSCs on B Cells
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Zhang Lei
Qu Yan-Nv
Zhang He-Yang
Wu Zhen-Yang
Li Zhong-Li
Guo Wan-Bei
Wang Qi-Ben
Fang Nan-Zhu
Jiang Xiao-Xia
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Abstract
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Background and Objectives: The effective use of MSCs for the treatment of some B cell-mediated immune diseases is quite limited. The main reason is that the immunomodulatory effects of mesenchymal stem cells (MSCs) on B cells are unclear, and their underlying mechanisms have not been fully explored.
Methods and Results: By co-culturing B cells with MSCs without (MSC/CTLsh) or with suppressor of cytokine signaling 1 (SOCS1) knockdown (MSC/SOCS1sh), we found that MSCs inhibited B cell proliferation, activation and terminal differentiation. Remarkably, the highest inhibition of B cell proliferation was observed in MSC/SOCS1sh co-culture. Besides, MSC/SOCS1sh reversed the inhibitory effect of MSCs in the last stage of B cell differentiation. However, MSC/SOCS1sh had no effect on inhibiting B cell activation by MSCs. We also showed that IgA+ B cell production was significantly higher in MSC/SOCS1sh than in MSC/CTLsh, although no difference was observed when both MSCs co-cultures were compared to isolated B cells. In addition, MSCs increased PGE2 production after TNF-α/IFN-γ stimulation, with the highest increase observed in MSC/SOCS1sh co-culture.
Conclusions: Our results highlighted the role of SOCS1 as an important new mediator in the regulation of B cell function by MSCs. Therefore, these data may help to develop new treatments for B cell-mediated immune diseases.
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KEYWORD
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MSCs immunomodulation, SOCS1, B cell
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