KMID : 1225120110030010051
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Vascular Neurology 2011 Volume.3 No. 1 p.51 ~ p.54
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Toxicologic Screening of Agmatine Using In Vitro and In Vivo Assays
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Jung Hyun-Joo
Jeon Yong-Heui Lee Jong-Eun
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Abstract
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Objective: To evaluate the both in vitro and in vivo toxicological effects of agmatine.
Methods: The toxicity of agmatine (50, 100 and 200 ¥ìM) was determined by using Alamar Blue assay in murine brain endothelial (bEnd.3) cells as vitro model and chick chorioallantoic membrane (CAM) assay as an in vivo model.
Results: The treatment of agmatine (50, 100 and 200 ¥ìM) showed no significant increase/decrease in the total cell number compared to control in the bEnd.3 cells in in vitro. The treatment of agmatine at three different concentrations (50, 100 and 200 ¥ìM) onto the CAM tissue for 2 days, showed similar survival rates of chick embryo to that of the control group.
Conclusions: The present results demonstrated that agmatine (50, 100 and 200 ¥ìM) doesn¡¯t exert any toxic effect as evidenced by the above results depicting the therapeutic potential of agmatine via modulation of vascularization for the treatment of neural diseases.
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KEYWORD
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Agmatine, Toxicology, Endothelial cell, Chick chorioallantoic membrane, Neurovascular unit
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