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KMID : 1236020160440020140
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Microbiology and Biotechnology Letters
2016 Volume.44 No. 2 p.140 ~ p.144
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Cloning of Chondroitinase ABC from Bacteroides stercoris HJ-15, a Human Intestinal Anaerobic Bacterium
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Bang Seo-Hyeon
Shim Ju-Won
Hyun Yang-Jin
Kim Dong-Hyun
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Abstract
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The gene encoding chondroitinase ABC from a genomic library of Bacteroides stercoris HJ-15, which was isolated from human feces, was cloned. The cloned gene consisted of 3,090 bp and was predicted to encode a 1,029?amino-acid protein. The B. stercoris chondroitinase ABC gene was not homologous to other chondroitinase ABC genes; however, its amino acid sequence showed 71% homology to that of Bacteroides thetaiotaomicron. The gene was cloned in the pET-26b+ expression vector and expressed under the T7 promoter in Escherichia coli BL21(DE3). The purified recombinant chondroitinase ABC degraded chondroitin sulfates A, B, and C.
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KEYWORD
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Chondroitinase ABC, Bacteroides stercoris HJ-15, Chondroitin sulfate, Cloning
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