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KMID : 1236020160440030268
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Microbiology and Biotechnology Letters
2016 Volume.44 No. 3 p.268 ~ p.277
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Anti-Inflammatory Effect of Chondrus ocellatus Holmes Ethanol Extract on Lipopolysaccharide-induced Inflammatory Responses in RAW 264.7 Cells
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Bae Nan-Young
Kim Min-Ji
Kim Koth-Bong-Woo-Ri
Park Ji-Hye
Park Sun-Hee
Sung Nak-Yun
Byun Eui-Hong
Ahn Dong-Hyun
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Abstract
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This study aimed to investigate the anti-inflammatory effect of the ethanol extract from Chondrus ocellatus Holmes (COHEE) in RAW 264.7 cells and in a mouse ear edema model, by measuring the production of lipopolysaccharide-induced inflammatory response mediators. There were no cytotoxic effects on the proliferation of macrophages treated with COHEE compared with the control. COHEE inhibited the production of nitric oxide and pro-inflammatory cytokines [interleukin (IL)-6, tumor necrosis factor-α, and IL-1β]. The extract also reduced the expression of inducible nitric oxide synthase, cyclooxygenase-2, nuclear factor-κB p65, and phosphorylated mitogen-activated protein kinase in a dose-dependent manner. In the croton-oil-induced ear edema model, COHEE decreased the formation of mouse ear edema at the highest dose compared with the control, and histological analysis revealed that the epidermal/dermal tissue thickness and mast cell numbers were reduced. Therefore, these results suggest that COHEE may be a promising topical anti-inflammatory therapeutic material through its action of modulating NF-κB and the MAPK signaling pathway.
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KEYWORD
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Chondrus ocellatus Holmes, anti-inflammatory effect, pro-inflammatory cytokines, NF-κB, MAPKs
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