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KMID : 1236020160440040535
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Microbiology and Biotechnology Letters
2016 Volume.44 No. 4 p.535 ~ p.539
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Inhibitory Effect of Transition Metal Gallium [Ga(NO3)3] on Biofilm Formation by Fish Pathogens
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Kim Dong-Hwi
Subramanian Dharaneedharan
Jang Young-Hwan
Heo Moon-Soo
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Abstract
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The prevalence of pathogenic bacteria such as Streptococcus parauberis (Sp), Streptococcus iniae (Si), and Edwardsiella tarda (Et) in flounder fish farms in Jeju Island and their management by gallium treatment was studied. Sp, Si, and Et were found to exhibit a low rate of cell growth and high biofilm formation. Hence, in the present study, cell growth and biofilm formation were measured spectrophotometrically 72 h after the addition of different concentrations of gallium (2, 4, or 8 mg/ml). In addition, cell death was measured by resazurin and propidium iodide staining assays. The results showed that bacterial cell death increased and biofilm formation decreased with an increasing concentration of gallium. Hence, the present study signifies that the use of gallium against bacterial pathogens could be useful for disease management in flounder farms.
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KEYWORD
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Biofilm, fish pathogen, gallium, olive flounder, propidium iodine, resazurin
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