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KMID : 1236020170450020162
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Microbiology and Biotechnology Letters
2017 Volume.45 No. 2 p.162 ~ p.167
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Bio-capsule Formation for Synchronous Saccharification and Fermentation Process
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Shin Gyeong-Yeon
Choi Hye-Jung
Kang Yang-Rae
Nam Ki-Du
Song Ju-Yeong
Joo Woo-Hong
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Abstract
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For the production of bioethanol by the synchronous saccharification and fermentation (SSF) process, biocapsule formation was attempted. Many saccharifying fungal strains and fermentative yeast strains were first screened. Aspergillus sp. BCNU 6200, Penicillium sp. BCNU 6201, and P. chrysogenum KACC 44363 were found to be excellent producers of saccharifying enzymes such as α-amylase and glucoamylase. Saccharomyces cerevisiae IFO-M-07 showed the highest ethanol productivity among the tested strains. Secondly, we determined the optimal conditions for pellet formation, and those for bio-capsule formation. All the tested fungal strains formed pellets, and the optimal conditions for bio-capsule formation were 28℃ and 120 rpm. Lastly, SSF process was performed using a bio-capsule. An ethanol yield of 3.9% was achieved by using the Aspergillus sp. BCNU 6200 bio-capsule (Aspergillus sp. BCNU 6200 + S. cerevisiae IFO-M-07) at 30℃ with shaking at 120 rpm during the 10 days of incubation. The results provide useful information on the application of a bio-capsule in bioethanol production under the SSF process.
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KEYWORD
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Bio-capsule, ethanol production, optimal conditions, saccharifying enzyme
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