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KMID : 1236020190470010064
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Microbiology and Biotechnology Letters
2019 Volume.47 No. 1 p.64 ~ p.68
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Changes in Isoflavone Contents and Physicochemical Properties of Soybean Leaf Extract by Extraction Conditions
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Yoon Jeong-Ah
Kwun Se-Young
Park Eun-Hee
Kim Myoung-Dong
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Abstract
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To utilize soybean leaf, which is a waste product in soybean production, as a value-added food material, this study aimed to investgate the extraction efficiency of isoflavone from soybean leaf and to characterize physicochemical properties of the extract. Maximum isoflavone content of 59.74 ± 4.54 mg/l was obtained from soybean leaf extracted at 90℃ for 12 h. DPPH (1,1-diphenyl-2-pricylhydrazyl) radical-scavenging activity and total polyphenol contents reached maximum levels of 67.26 ± 3.64% and 1,688.68 ± 97.37 μg/ml chlorogenic acid equivalent, respectively. Based on the contents of isoflavone and total polyphenol, and DPPH radical-scavenging activity, optimum extraction conditions for soybean leaf using water as solvent were 90℃ and 12 h.
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KEYWORD
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Soy bean leaf, isoflavone, DPPH radical-scavenging activity, polyphenol, extraction
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