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KMID : 1236020190470010148
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Microbiology and Biotechnology Letters
2019 Volume.47 No. 1 p.148 ~ p.157
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A Study on the Whitening Effect of Erigeron annuus (L.) Pers. Ethanol Extract on Melanoma Cell (B16F10)
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Joo Da-Hye
Yoo Dan-Hee
Lee Jin-Young
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Abstract
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A 70% ethanol extract of Erigeron annuus (L.) Pers. was investigated for its whitening activity for application as a functional ingredient in cosmetic products. At the E. annuus extract concentration of 1,000 μg/ml, the electron-donating ability was found to be 67.83%, the tyrosinase inhibitory effect (related to skin-whitening) was 69%, the elastase inhibitory effect (related to skin-wrinkling) was 69%, and the astringent effect was 80%. The ABTS+ radical-scavenging ability was 87% at the 500 μg/ml concentration. In the cell viability test measured on melanoma cells, 96% of the cells treated with 100 μg/ml of the extract were viable. According to the western blot results, the protein expression of the microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein (TRP) -1, and TRP-2 was decreased by 60.22%, 47.83%, 54.79%, and 67.88%, respectively, at the extract concentration of 100 μg/ml. The protein expression of phosphorylated extracellular signal regulated kinase (p-ERK) and phosphorylated cAMP response elementbinding protein (p-CREB) was decreased with increasing concentrations of the extract. Reverse transcription- polymerase chain reaction of the extract showed that the mRNA expression of MITF, tyrosinase, TRP- 1, and TRP-2 was decreased by 86.51%, 85.22%, 74.26%, and 66.66%, respectively, at 100 μg/ml extract concentration. The findings suggest that the 70% ethanol extract from E. annuus (L.) Pers. has potential as a cosmeceutical ingredient with whitening effect.
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KEYWORD
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Erigeron annuus (L.) Pers., whitning effect, melanoma cell, western blot, RT-PCR
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