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KMID : 1236020190470030434
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Microbiology and Biotechnology Letters
2019 Volume.47 No. 3 p.434 ~ p.440
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Exploration of β-Glucuronidase Activity of Lactic Acid Bacteria Isolated from Kimchi
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Kim Eun-Jung
Shin In-Ung
Kwun Se-Young
Park Eun-Hee
Lee Jae-Hyoung
Kim Myoung-Dong
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Abstract
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Lactic acid bacteria (LAB) isolated from kimchi were studied for their β-glucuronidase activity. Among the 156 strains tested, 52 strains utilized glucuronic acid as a carbon source and their intracellular β-glucuronidase activities were significantly higher than their extracellular activities. Leuconostoc mesenteroides KFRI 73007 isolated from turnip kimchi exhibited the highest intracellular β-glucuronidase activity of 0.77 ± 0.01 U/mg protein, which was further increased to 1.14 ± 0.01 U/mg protein under optimized reaction conditions (pH 7, 37℃). The activity of β-glucuronidase was notably decreased by the addition of divalent cations, and glucuronic acid was the best carbon source to produce β-glucuronidase in Leu. mesenteroides KFRI 73007.
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KEYWORD
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β-Glucuronidase, Kimchi, Lactic acid bacteria, Leuconostoc mesenteroides
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