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KMID : 1236020200480020179
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Microbiology and Biotechnology Letters
2020 Volume.48 No. 2 p.179 ~ p.184
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Isolation of Ethanol-producing Thermotolerant Yeast Hanseniaspora opuntiae from Senecio cruentus
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Yoon Jeong-Ah
Do Young-Eun
Park Eun-Hee
Bae Young-Woo
Kim Myoung-Dong
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Abstract
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The MBY/L6793 strain showing the highest ethanol yield of 0.48 ± 0.00 g ethanol/ g glucose was isolated from Senecio cruentus. Its ethanol yield was approximately 1.5 times that of the MBY/L6986 isolated from Callistephus chinensis. The strain was identified as Hanseniaspora opuntiae by sequence analysis of the 18S rRNA gene, and the sequenced gene was registered to the GenBank (MN859968). When grown at 40℃, the strain produced 3.82 ± 0.98 g ethanol from 20 g glucose and 10.05 ± 0.06 g ethanol from 60 g glucose, corresponding to approximately 2.45 and 5.74 times, respectively, compared to the control strain H. opuntiae KCCM50747. The MBY/L6793 strain was deposited to KCTC (Korean Collection for Type Culture) as KCTC37025.
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KEYWORD
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Ethanol, yeast, thermotolerance, flower, Hanseniaspora opuntiae
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