|
KMID : 1236020200480030276
|
|
Microbiology and Biotechnology Letters
2020 Volume.48 No. 3 p.276 ~ p.286
|
|
|
Probiotic Potential of Pediococcus acidilactici and Enterococcus faecium Isolated from Indigenous Yogurt and Raw Goat Milk
|
|
Sarkar Shovon Lal
Hossain Md. Iqbal
Monika Sharmin Akter
Sanyal Santonu Kumar
Roy Pravas Chandra
Hossain Md. Anwar
Jahid Iqbal Kabir
|
|
|
Abstract
|
|
|
|
Probiotics are live microorganisms that, when administered in adequate amounts, confer health benefits to the host. This study was conducted for the isolation of potential lactic acid bacteria (LAB) with probiotic properties from goat milk and yogurt. Several tests were conducted in vitro using the standard procedures for evaluating the inhibitory spectra of LAB against pathogenic bacteria; tolerance to NaCl, bile salt, and phenol; hemolytic, milk coagulation, and bile salt hydrolase activities; gastrointestinal transit tolerance; adhesion properties; and antibiotic susceptibility. Among 40 LAB strains screened according to culture characteristics, five isolates exhibited antagonistic properties. Three were identified as Pediococcus acidilactici, and two were identified as Enterococcus faecium, exploiting 16S rRNA gene sequencing. All the isolates succeeded in the gastrointestinal transit tolerance assay and successively colonized mucosal epithelial cells. Based on the results of these in vitro assays, both P. acidilactici and E. faecium can be considered as potential probiotic candidates.
|
|
|
KEYWORD
|
|
|
Probiotic, antagonistic assay, Pediococcus acidilactici, Enterococcus faecium, goat milk, 16s rRNA
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|