KMID : 1236020200480030358
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Microbiology and Biotechnology Letters 2020 Volume.48 No. 3 p.358 ~ p.365
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Biochemical Characterization of a Novel Alkaline and Detergent Stable Protease from Aeromonas veronii OB3
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Manni Laila
Misbah Asmae Zouine Nouhaila Ananou Samir
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Abstract
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An organic solvent- and bleach-stable protease-producing strain was isolated from a polluted river water sample and identified as Aeromonas veronii OB3 on the basis of biochemical properties (API 20E) and 16S rRNA sequence analysis. The strain was found to hyper-produce alkaline protease when cultivated on fish waste powder-based medium (HVSP, 4080 U/ml). The biochemical properties and compatibility of OB3 with several detergents and additives were studied. Maximum activity was observed at pH 9.0 and 60¡É. The crude protease displayed outstanding stability to the investigated surfactants and oxidants, such as Tween 80, Triton X-100, and H2O2, and almost 36% residual activity when incubated with 1% SDS. Remarkably, the enzyme demonstrated considerable compatibility with commercial detergents, retaining more than 100% of its activity with Ariel and Tide (1 h, 40¡É). Moreover, washing performance of Tide significantly improved by the supplementation of small amounts of OB3 crude protease. These properties suggest the potential use of this alkaline protease as a bio-additive in the detergent industry and other biotechnological processes such as peptide synthesis.
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KEYWORD
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Protease, alkaline, Aeromonas veronii, detergent, solvent-stable, fish waste medium
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