|
KMID : 1239920070010010019
|
|
Nutrition Research and Practice
2007 Volume.1 No. 1 p.19 ~ p.28
|
|
|
Transcriptome analysis and promoter sequence studies on early adipogenesis in 3T3-L1 cells
|
|
Kim Su-Jong
Cha Youn-Soo
Kwon Dae-Young
Lee Yong-Sung
Mun Eun-Gyeng
Lee Ki-Hwan
|
|
|
Abstract
|
|
|
To identify regulatory molecules which play key roles in the development of obesity, we investigated the transcriptional profiles in 3T3-L1 cells at early stage of differentiation and analyzed the promoter sequences of differentially regulated genes. One hundred and sixty-one (161) genes were found to have significant changes in expression at the 2nd day following treatment with differentiation cocktail. Among them, 86 transcripts were up-regulated and 75 transcripts were down-regulated. The 161 transcripts were classified into 10 categories according to their functional roles; cytoskeleton,
cell adhesion, immune, defense response, metabolism, protein modification, protein metabolism, regulation of transcription, signal transduction and transporter. To identify transcription factors likely involved in regulating these differentially expressed genes, we analyzed the promoter sequences of up- or ?down regulated genes for the presence of transcription factor binding sites (TFBSs). Based on coincidence of regulatory sites, we have identified candidate transcription factors (TFs), which include those previously known to be involved in adipogenesis (CREB, OCT-1 and c-Myc). Among them, c-Myc was also identified by our microarray data. Our approach to take advantage of the resource of the human genome
|
|
|
KEYWORD
|
|
|
Microarray analysis, promoter analysis, adipocyte differentiation, 3T3-L1 cells, transcription factor binding sites
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
  
|
|