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KMID : 1239920090030030180
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Nutrition Research and Practice
2009 Volume.3 No. 3 p.180 ~ p.184
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β-Glucan enhanced apoptosis in human colon cancer cells SNU-C4
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Kim Mi-Ja
Hong Se-Young
Kim Sun-Kyu
Cheong Chul
Park Hong-Ju
Chun Hye-Kyung
Jang Ki-Hyo
Yoon Byung-Dae
Kim Chul-Ho
Kang Soon-Ah
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Abstract
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The apoptotic effect of bacteria-derived β-glucan was investigated in human colon cancer cells SNU-C4 using terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, reverse transcription-polymerase chain reaction (RT-PCR) expressions of Bcl-2, Bax, and Caspase-3 genes, and assay of caspase-3 enzyme activity. β-Glucan of 10, 50, and 100 μg/mL decreased cell viability in a dose-dependent manner with typical apoptotic characteristics, such as morphological changes of chromatin condensation and apoptotic body formation from TUNEL assay. In addition, β-glucan (100 μg/mL) decreased the expression of Bcl-2 by 0.6 times, whereas the expression of Bax and Caspase-3 were increased by 3.1 and 2.3 times, respectively, compared to untreated control group. Furthermore, the caspase-3 activity in the β-glucan-treated group was significantly increased compared to those in control group (P < 0.05). Bacterial derived β-glucan could be used as an effective compound inducing apoptosis in human colon cancer.
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KEYWORD
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β-glucan, apoptosis, colon cancer, caspase-3
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