|
KMID : 1239920110050020112
|
|
Nutrition Research and Practice
2011 Volume.5 No. 2 p.112 ~ p.116
|
|
|
Effect of paternal folate deficiency on placental folate content and folate receptor α expression in rats
|
|
Kim Hye-Won
Choi Yun-Jung
Kim Ki-Nam
Tamura Tsunenobu
Chang Nam-Soo
|
|
|
Abstract
|
|
|
|
We investigated the effect of paternal folate status on folate content and expression of the folate transporter folate receptor α (FRα) in rat placental tissues. Rats were mated after males were fed a diet containing 0 mg of folic acid/kg of diet (paternal folate-deficient, PD) or 8 mg folic acid/kg of diet (paternal folate-supplemented, PS) for 4 weeks. At 20 days of gestation, the litter size, placental weight, and fetal weight were measured, and placental folate content (n = 8/group) and expression of FRα (n = 10/group) were analyzed by microbiological assay and Western blot analysis, respectively. Although there was no difference observed in litter size or fetal weight, but significant reduction (10%) in the weight of the placenta was observed in the PD group compared to that in the PS group. In the PD group, placental folate content was significantly lower (by 35%), whereas FRα expression was higher (by 130%) compared to the PS group. Our results suggest that paternal folate status plays a critical role in regulating placental folate metabolism and transport.
|
|
|
KEYWORD
|
|
|
Paternal folate status, placental folate, placental weight, folate receptor alpha
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
  
|
|