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KMID : 1239920160100030259
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Nutrition Research and Practice
2016 Volume.10 No. 3 p.259 ~ p.264
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Compound K attenuates stromal cell-derived growth factor 1 (SDF-1)-induced migration of C6 glioma cells
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Kim Hyuck
Roh Hyo-Sun
Kim Jai-Eun
Park Sun-Dong
Park Won-Hwan
Moon Jin-Young
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Abstract
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BACKGROUND/OBJECTIVES: Stromal cell-derived growth factor 1 (SDF-1), also known as chemokine ligand 12, and chemokine receptor type 4 are involved in cancer cell migration. Compound K (CK), a metabolite of protopanaxadiol-type ginsenoside by gut microbiota, is reported to have therapeutic potential in cancer therapy. However, the inhibitory effect of CK on SDF-1 pathway-induced migration of glioma has not yet been established.
MATERIALS/METHODS: Cytotoxicity of CK in C6 glioma cells was determined using an EZ-Cytox cell viability assay kit. Cell migration was tested using the wound healing and Boyden chamber assay. Phosphorylation levels of protein kinase C (PKC)α and extracellular signal-regulated kinase (ERK) were measured by western blot assay, and matrix metallopeptidases (MMP) were measured by gelatin-zymography analysis.
RESULTS: CK significantly reduced the phosphorylation of PKCα and ERK1/2, expression of MMP9 and MMP2, and inhibited the migration of C6 glioma cells under SDF-1-stimulated conditions.
CONCLUSIONS: CK is a cell migration inhibitor that inhibits C6 glioma cell migration by regulating its downstream signaling molecules including PKCα, ERK1/2, and MMPs.
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KEYWORD
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Panax ginseng, compound K, SDF-1, anti-tumor, C6 glioma
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