KMID : 1239920170110020090
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Nutrition Research and Practice 2017 Volume.11 No. 2 p.90 ~ p.96
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Anti-inflammatory effect of lycopene in SW480 human colorectal cancer cells
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Cha Jae-Hoon
Kim Woo-Kyoung Ha Ae-Wha Kim Myung-Hwan Chang Moon-Jeong
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Abstract
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BACKGROUND/OBJECTIVES: Although the antioxidative effects of lycopene are generally known, the molecular mechanisms underlying the anti-inflammatory properties of lycopene are not fully elucidated. This study aimed to examine the role and mechanism of lycopene as an inhibitor of inflammation.
METHODS/MATERIALS: Lipopolysaccharide (LPS)-stimulated SW 480 human colorectal cancer cells were treated with 0, 10, 20, and 30 ¥ìM lycopene. The MTT assay was performed to determine the effects of lycopene on cell proliferation. Western blotting was performed to observe the expression of inflammation-related proteins, including nuclear factor-kappa B (NF-¥êB), inhibitor kappa B (I¥êB), mitogen-activated protein kinase (MAPK), extracellular signal-related kinase (ERK), c-jun NH2-terminal kinase (JNK), and p38 (p38 MAP kinase). Real-time polymerase chain reaction was performed to investigate the mRNA expression of tumor necrosis factor ¥á (TNF-¥á), interleukin-1 beta (IL-1¥â), interleukin-6 (IL-6), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). Concentrations of nitric oxide (NO) and prostaglandin E2 (PGE2) were determined via enzyme-linked immunosorbent assays.
RESULTS: In cells treated with lycopene and LPS, the mRNA expression of TNF-¥á, IL-1¥â, IL-6, iNOS, and COX-2 were decreased significantly in a dose-dependent manner (P < 0.05). The concentrations of PGE2 and NO decreased according to the lycopene concentration (P < 0.05). The protein expressions of NF-¥êB and JNK were decreased significantly according to lycopene concertation (P < 0.05).
CONCLUSIONS: Lycopene restrains NF-¥êB and JNK activation, which causes inflammation, and suppresses the expression of TNF-¥á, IL-1¥â, IL-6, COX-2, and iNOS in SW480 human colorectal cancer cells.
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KEYWORD
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Lycopene, colorectal cancer, inflammation, nitric oxide, prostaglandin
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