KMID : 1239920170110020121
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Nutrition Research and Practice 2017 Volume.11 No. 2 p.121 ~ p.129
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Effects of dietary leucine supplementation on the hepatic mitochondrial biogenesis and energy metabolism in normal birth weight and intrauterine growth-retarded weanling piglets
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Su Weipeng
Xu Wen Zhang Hao Ying Zhixiong Zhou Le Zhang Lili Wang Tian
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Abstract
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BACKGROUND/OBJECTIVES: The study was conducted to evaluate the effects of dietary leucine supplementation on mitochondrial biogenesis and energy metabolism in the liver of normal birth weight (NBW) and intrauterine growth-retarded (IUGR) weanling piglets.
MATERIALS/METHODS: A total of sixteen pairs of NBW and IUGR piglets from sixteen sows were selected according to their birth weight. At postnatal day 14, all piglets were weaned and fed either a control diet or a leucine-supplemented diet for 21 d. Thereafter, a 2 ¡¿ 2 factorial experimental design was used. Each treatment consisted of eight replications with one piglet per replication.
RESULTS: Compared with NBW piglets, IUGR piglets had a decreased (P < 0.05) hepatic adenosine triphosphate (ATP) content. Also, IUGR piglets exhibited reductions (P < 0.05) in the activities of hepatic mitochondrial pyruvate dehydrogenase (PDH), citrate synthase (CS), ¥á-ketoglutarate dehydrogenase (¥á-KGDH), malate dehydrogenase (MDH), and complexes I and V, along with decreases (P < 0.05) in the concentration of mitochondrial DNA (mtDNA) and the protein expression of hepatic peroxisome proliferator-activated receptor-¥ã coactivator 1¥á (PGC-1¥á). Dietary leucine supplementation increased (P < 0.05) the content of ATP, and the activities of CS, ¥á-KGDH, MDH, and complex V in the liver of piglets. Furthermore, compared to those fed a control diet, piglets given a leucine-supplemented diet exhibited increases (P < 0.05) in the mtDNA content and in the mRNA expressions of sirtuin 1, PGC-1¥á, nuclear respiratory factor 1, mitochondrial transcription factor A, and ATP synthase, H+ transporting, mitochondrial F1 complex, ¥â polypeptide in liver.
CONCLUSIONS: Dietary leucine supplementation may exert beneficial effects on mitochondrial biogenesis and energy metabolism in NBW and IUGR weanling piglets.
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KEYWORD
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Leucine, intrauterine growth retardation, weanling piglets, mitochondrial biogenesis, energy metabolism
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