|
KMID : 1239920170110040265
|
|
Nutrition Research and Practice
2017 Volume.11 No. 4 p.265 ~ p.274
|
|
|
Anti-inflammatory effects of Nelumbo leaf extracts and identification of their metabolites
|
|
Park Eun-Kyo
Kim Gyoung-Deuck
Go Min-Sun
Kwon Do-Dan
Jung In-Kyung
Auh Joong-Hyuck
Kim Jung-Hyun
|
|
|
Abstract
|
|
|
BACKGROUND/OBJECTIVES: Nelumbo leaves have been used in traditional medicine to treat bleeding, gastritis, hemorrhoids, and halitosis. However, their mechanisms have not been elucidated.
MATERIALS/METHODS: The present study prepared two Nelumbo leaf extracts (NLEs) using water or 50% ethanol. Inflammatory response was induced with LPS treatment, and expression of pro-inflammatory mediators (inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6 and nitric oxide (NO) and prostaglandin E2 (PGE2) productions were assessed. To determine the anti-inflammatory mechanism of NLEs, we measured nuclear factor-κB (NF-κB) activity. Major metabolites of NLEs were also analyzed and quantified.
RESULTS: NLEs effectively reduced the expression and productions of pro-inflammatory mediators such as IL-1β, IL-6, TNF-α, PGE2, and NO. NLEs also reduced NF-κB activity by inhibiting inhibitor of NF-κB phosphorylation. Both extracts contained catechin and quercetin, bioactive compounds of NLEs.
CONCLUSIONS: In this study, we showed that NLEs could be used to inhibit NF-κB-mediated inflammatory responses. In addition, our data support the idea that NLEs can ameliorate disease conditions involving chronic inflammation.
|
|
|
KEYWORD
|
|
|
Nelumbo leaf, inflammation, metabolomics, macrophage
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
  
|
|