KMID : 1239920190130040279
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Nutrition Research and Practice 2019 Volume.13 No. 4 p.279 ~ p.285
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Protective effect of Cordyceps militaris against hydrogen peroxide-induced oxidative stress in vitro
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He Mei Tong
Lee Ah-Young Park Chan-Hum Cho Eun-Ju
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Abstract
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BACKGROUND/OBJECTIVES: Excessive production of reactive oxygen species (ROS) such as hydroxyl (¡¤OH), nitric oxide (NO), and hydrogen peroxide (H2O2) is reported to induce oxidative stress. ROS generated by oxidative stress can potentially damage glial cells in the nervous system. Cordyceps militaris (CM), a kind of natural herb widely found in East Asia. In this study, we investigated the free radical scavenging activity of the CM extract and its neuroprotective effects in H2O2-induced C6 glial cells.
MATERIALS/METHODS: The ethanol extract of CM (100?1,000 ¥ìg/mL) was used to measure DPPH, ¡¤OH, and NO radical scavenging activities. In addition, hydrogen peroxide (H2O2)-induced C6 glial cells were treated with CM at 0.5?2.5 ¥ìg/mL for measurement of cell viability, ROS production, and protein expression resulting from oxidative stress.
RESULTS: The CM extract showed high scavenging activities against DPPH, ¡¤OH, and NO radicals at concentration of 1,000 ¥ìg/mL. Treatment of CM with H2O2-induced oxidative stress in C6 glial cells significantly increased cell viability, and decreased ROS production. Cyclooxygenase-2 and inducible nitric oxide synthase protein expression was down-regulated in CM-treated groups. In addition, the protein expression level of phospho-p38 mitogen-activated protein kinase (p-p38 MAPK), phospho-c-Jun N-terminal kinase (p-JNK), and phospho-extracellular regulated protein kinases (p-ERK) in H2O2-induced C6 glial cells was down-regulated upon CM administration.
CONCLUSION: CM exhibited radical scavenging activity and protective effect against H2O2 as indicated by the increased cell viability, decreased ROS production, down-regulation of inflammation-related proteins as well as p-p38, p-JNK, and p-ERK protein levels. Therefore, we suggest that CM could play the protective role from oxidative stress in glial cells.
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KEYWORD
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Cordyceps militaris, free radicals, neuroglia, hydrogen peroxide, oxidative stress
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