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KMID : 1239920230170050844
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Nutrition Research and Practice
2023 Volume.17 No. 5 p.844 ~ p.854
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Antimetastatic effect of fucoidan against non-small cell lung cancer by suppressing non-receptor tyrosine kinase and extracellular signal-related kinase pathway
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Nareenath Muneerungsee
Supita Tanasawet
Wanida Sukketsiri
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Abstract
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BACKGROUND/OBJECTIVES : Fucoidan, a polysaccharide content in brown algae, has been reported to inhibit the growth of cancer cells. The present study aimed to investigate the suppression effects of fucoidan on A549 non-small cell lung cancer cells migration.
MATERIALS/METHODS : The anti-migratory activity of fucoidan in A549 cells was examined by wound healing assay and phalloidin-rhodamine staining in response to fucoidan (0?100 μg/mL) treatment for 48 h. Western blot analysis was performed to clarify the protein expressions relevant to migratory activity.
RESULTS : Fucoidan (25?100 μg/mL) significantly suppressed A549 cells migration together with reduced the intensity of phalloidin-rhodamine which detect filopodia and lamellipodia protrusions at 48 h of treatment. The protein expression indicated that fucoidan significantly suppressed the phosphorylation of focal adhesion kinase (FAK), Src, and extracellular signal-related kinase (ERK). In addition, the phosphorylation of p38 in A549 cells was found to be increased.
CONCLUSIONS : Our data conclude that fucoidan exhibits anti-migratory activities against lung cancer A549 cells mediated by inhibiting ERK1/2 and FAK-Src pathway.
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KEYWORD
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Fucoidan, non-small cell lung cancer, filopodia, metastasis
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