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KMID : 1239920230170061043
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Nutrition Research and Practice
2023 Volume.17 No. 6 p.1043 ~ p.1055
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Cydonia oblonga Miller fruit extract exerts an anti-obesity effect in 3T3-L1 adipocytes by activating the AMPK signaling pathway
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Lee Hyun-Sook
Jung Jae-In
Hwang Jung-Soon
Hwang Myeong-Oh
Kim Eun-Ji
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Abstract
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BACKGROUND/OBJECTIVES : The fruit of Cydonia oblonga Miller (COM) is used traditionally in Mediterranean region medicine to prevent or treat obesity, but its mechanism of action is still unclear. Beyond a demonstrated anti-obesity effect, the fruit was tested for the mechanism of adipogenesis in 3T3-L1 preadipocytes.
MATERIALS/METHODS : 3T3-L1 preadipocytes were cultured for 8 days with COM fruit extract (COME) at different concentrations (0?600 μg/mL) with adipocyte differentiation medium. The cell viability was measured using an MTT assay; triglyceride (TG) was stained with Oil Red O. The expression levels of the adipogenesis-related genes and protein expression were analyzed by reverse transcription polymerase chain reaction and Western blotting, respectively.
RESULTS : COME inhibited intracellular TG accumulation during adipogenesis. A COME treatment in 3T3-L1 cells induced upregulation of the adenosine monophosphate-activated protein kinase (AMPK)α phosphorylation and downregulation of the adipogenic transcription factors, such as sterol regulatory element-binding protein 1c, peroxisome proliferator-activated receptor γ, and CCAAT/enhancer binding protein α. The COME treatment reduced the mRNA expression of fatty acyl synthetase, adenosine triphosphate-citrate lyase, adipocyte protein 2, and lipoprotein lipase. It increased the mRNA expression of hormone-sensitive lipase and carnitine palmitoyltransferase I in 3T3-L1 cells.
CONCLUSIONS : COME inhibits adipogenesis via the AMPK signaling pathways. COME may be used to prevent and treat obesity.
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KEYWORD
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3T3-L1 cells, AMP-activated protein kinase, adipogenesis, lipogenesis, lipolysis
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