KMID : 1240020180220000139
|
|
International Neurourology Journal 2018 Volume.22 No. 0 p.139 ~ p.146
|
|
Dexmedetomidine Ameliorates Sleep Deprivation-Induced Depressive Behaviors in Mice
|
|
Moon Eun-Jin
Ko Il-Gyu Kim Sung-Eun Jin Jun-Jang Hwang Lak-Kyong Kim Chang-Ju An Hyeon-Jun Lee Bong-Jae Yi Jae-Woo
|
|
Abstract
|
|
|
Purpose: Sleep deprivation induces depressive symptoms. Dexmedetomidine is a ¥á2-adrenoreceptor agonist and this drug possesses sedative, anxiolytic, analgesic, and anesthetic-sparing effect. In this study, the action of dexmedetomidine on sleep deprivation-induced depressive behaviors was investigated using mice.
Methods: For the inducing of sleep deprivation, the mice were placed inside a water cage containing 15 platforms and filled with water up to 1 cm below the platform surface for 7 days. One day after sleep deprivation, dexmedetomidine at the respective dosage (0.5, 1, and 2 ¥ìg/kg) was intraperitoneally treated into the mice, one time per a day during 6 days. Then, forced swimming test and tail suspension test were conducted. Immunohistochemistry for tyrosine hydroxylase (TH), 5-hydroxytryptamine (5-HT; serotonin), tryptophan hydroxylase (TPH) and western blot for D1 dopamine receptor were also performed.
Results: Sleep deprivation increased the immobility latency in the forced swimming test and tail suspension test. The expressions of TPH, 5-HT, and D1 dopamine receptor were decreased, whereas, TH expression was increased by sleep deprivation. Dexmedetomidine decreased the immobility latency and increased the expressions of TPH, 5-HT, and D1 dopamine receptor, whereas, HT expression was decreased by dexmedetomidine treatment.
Conclusions: In our results, dexmedetomidine alleviated sleep deprivation-induced depressive behaviors by increasing 5-HT synthesis and by decreasing dopamine production with up-regulation of D1 dopamine receptor.
|
|
KEYWORD
|
|
Sleep deprivation, Dexmedetomidine, Depression, Serotonin, Dopamine
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|