KMID : 1377020190160060605
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Tissue Engineering and Regenerative Medicine 2019 Volume.16 No. 6 p.605 ~ p.614
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Evaluation of Polycaprolactone-Associated Human Nasal Chondrocytes as a Therapeutic Agent for Cartilage Repair
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Kim Do-Hyun
Lim Mi-Hyun Jeun Jung-Ho Park Sun-Hwa Lee Weon-Sun Park Sang-Hi Kwon Mi-Yeon Hwang Se-Hwan Kim Sung-Won
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Abstract
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Background: In this study, we manufactured a complex of human nasal septal cartilage (hNC) with polycaprolactone (PCL) for transplantation into cartilaginous skeletal defects and evaluated their characteristics.
Methods: Nasal septum tissue was obtained from five patients aged ¡Ã?20 years who were undergoing septoplasty. hNCs were isolated and subcultured for three passages in vitro. To formulate the cell?PCL complex, we used type I collagen as an adhesive between chondrocyte and PCL. Immunofluorescence staining, cell viability and growth in the hNC?PCL complex, and mycoplasma contamination were assessed.
Results: hNCs in PCL showed viability ¡Ã?70% and remained at these levels for 9 h of incubation at 4 ¡ÆC. Immunostaining of the hNC?PCL complex also showed high expression levels of chondrocyte-specific protein, COL2A1, SOX9, and aggrecan during 24 h of clinically applicable conditions.
Conclusion: The hNC?PCL complex may be a valuable therapeutic agent for implantation into injured cartilage tissue, and can be used clinically to repair cartilaginous skeletal defects. From a clinical perspective, it is important to set the short duration of the implantation process to achieve effective functional implantation.
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KEYWORD
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Chondrocyte, Collagen, Human nasal septum, Tissue engineering, Cartilage
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