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KMID : 1813520180070010001
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Journal of the Korean Glaucoma Society
2018 Volume.7 No. 1 p.1 ~ p.11
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Neuroprotective Effect of Resveratrol via Sirtuin 1 Pathway in a Rat Model of Chronic Ocular Hypertension
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Heo Hwan
Sung Mi-Sun
Park Sang-Woo
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Abstract
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Purpose : To investigate the neuroprotective effect and to determine the neuroprotective mechanisms of resveratrol for retinal ganglion cells (RGC) in a rat model of chronic intraocular pressure (IOP) elevation.
Methods : Three-month-old Sprague-Dawley rats received saline or resveratrol (10 mg/kg) intraperitoneally once daily during the chronic IOP elevation period. Sirtuin 1 (SIRT1), peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α), and glial fibrillary acidic protein (GFAP) expression were assessed at 1, 2, 3, and 4 weeks following onset of chronic IOP elevation. The effects of resveratrol on SIRT1, PGC-1α, and GFAP expression were assessed in retinas of rats treated with saline or resveratrol at 3 weeks after chronic IOP elevation. RGC survival was assessed by labeling flat-mounted retinas with Brn3a at 4 weeks after chronic IOP elevation.
Results : SIRT1 and PGC-1α expression were downregulated in retinas from saline treated rats. This downregulation was reversed by resveratrol treatment, resulting in a significant difference between groups at 3 weeks following chronic IOP elevation. GFAP expression was upregulated in retinas of saline treated rats and suppressed by resveratrol treatment. Resveratrol treatment also protected against RGC loss at 4 weeks following chronic IOP elevation.
Conclusions : Our results highlight that resveratrol might be a potential neuroprotective agent against RGC loss via SIRT1 pathway under chronic IOP elevation conditions.
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KEYWORD
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Neuroprotection, Sirtuin 1, Ocular hypertension
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