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KMID : 1813520200090010001
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Journal of the Korean Glaucoma Society
2020 Volume.9 No. 1 p.1 ~ p.9
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Effect of Plant-derived Angiogenin Fusion Protein's on Matrix Metalloproteinase-1 and Alpha Smooth Muscle Actin in Trabecular Meshwork Cells
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Jeong Jae-Hoon
Ko Ki-Sung
Lee Jeong-Hwan
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Abstract
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Purpose : Benzalkonium chloride (BAK), commonly used in glaucoma treatment, is an eye drop preservative with dose-dependent toxicity. Previous studies have observed the multi-functional benefits of angiogenin (ANG) against glaucoma. In our study, we developed a plant-derived ANG fusion protein and evaluated its effect on the trabecular meshwork (TM) damage induced by BAK in human TM cell lines.
Methods : Plant-derived ANG (ANG-FcK) was synthesized by fusing immunoglobulin G's Fc region and KDEL to conventional recombinant human ANG (Rh-ANG) purified from transgenic tobacco plants. Expression patterns of matrix metalloproteinase-1 (MMP-1) and alpha smooth muscle actin (a-SMA) in human TM cell lines were evaluated by immunocytochemical analysis and at the levels of protein and mRNA. We used BAK to look for toxic changes, and ANG-FcK and Rh-ANG for the protective effects.
Results : In human TM cell lines, BAK induced more prominent expressions of MMP-1 and α-SMA. ANG co-treatment augmented the MMP-1 expression, however lessened the α-SMA expression. Expression were showed similar pattern between the ANG-FcK and Rh-ANG.
Conclusions : Plant-derived ANG-FcK showed similar effects as Rh-ANG in the BAK-induced degenerative TM cell lines, and that effect might be related to the anti-fibrosis mechanism, suggesting that plant-derived ANG-FcK could be a promising glaucoma treatment.
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KEYWORD
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Angiogenin, Benzalkonium chloride, Fibrosis, Molecular farming, Trabecular meshwork
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