In order to investigate the effects of Gylkyungtang and Gylkyungtang-gamibang on antitumor effects after Sarcoma 180 cells transplantation into the peritoneal cavity or left groin in mice, and immune depression in mice induced by methotrexate, the extracts of its herbal medicines were orally administered for 14 or 21 days.
Experimental studies were performed for measureance of mean survival days, tumor weight for antitumor effects, white blood cell, T-cell and B-cell quantitation, delayed type hypersensitivity, hemagglutinine titer, hemolysin titer, rosette forming cells, lymphocyte transformation, plaque forming cells, interleukin-2 productivity, natural killer cell activity and phagocytic activity for immune responses in the immune depressed ICR mice.
The results were obtained as follows :
1. Mean survival time in Gylkyungtang and Gylkyungtanggamibang-treated groups were increased with the statistical significance, as compared with the control group.
2. Tumor weights in Gylkyungtang and Gylkyungtanggamibang-treated groups were decreased with the statistical significance, as compared with the control group.
3. White blood cell in Gylkyungtang and Gylkyungtanggamibang-treated groups were increased with the statistical significance, as compared with the control group.
4. T-cell quauntitation in Gylkyungtang and Gylkyungtanggamibang-treated groups were slightly increased with no effectiveness, as compared with the control group.
5. B-cell quauntitation in Gylkyungtang-treated groups was increased with the statistical significance but Gylkyungtanggamibang-treated group was slightly increased with no effectiveness, as compared with the control group.
6. Delayed type hypersensitivity in Gylkyungtang and Gylkyungtanggamibang-treated groups were slightly increased with no effectiveness, as compared with the control group.
7. Hemagglutinin titer in Gylkyungtang and Gylkyungtanggamibang-treated groups were increased with the statistical significance, as compared with the control group.
8. Hemolysin titer in Gylkyungtang and Gylkyungtanggamibang-treated groups were increased with the statistical significance, as compared with the control group.
9. Rosette forming cells in Gylkyungtang and Gylkyungtanggamibang-treated groups were slightly increased with no effectiveness, as compared with the control group.
10. Lymphocyte transfomation in Gylkyungtang and Gylkyungtanggamibang-treated groups were slightly increased with no effectiveness, as compared with the control group.
11. Plaque forming cells in Gylkyungtang and Gylkyungtanggamibang-treated groups were slightly increased with no effectiveness, as compared with the control group.
12. Interleukin-2 productivity in Gylkyungtang-treated group was with slightly increased with no effectiveness but Gylkyungtanggamibang-treated group was increased with the statistical significance, as compared with the control group.
13. Natural killer cell activity in Gylkyungtang-treated group at E/T ratio 50 : 1 was increased with statistical significance but Gylkyungtanggamibang-treated group was slightly increased with no effectiveness, as compared with the control group.
14. Phagocytic activity in Gylkyungtang and Gylkyungtanggamibang-treated groups were increased with the statistical significance, as compared with the control group.
According to the above results, it could be suggested that Gylkyungtang and Gylkyungtanggamibang have prominent antitumor effects, enhance effect both cellular and humoral immunity.
|