In order to investigate the effects of bee venom aqua-acupuncture(BVA) on the immunosuppression induced by methotrexate(MTX), three different products with 0.1ml of 0.035%(0.7mg/2ml) of bee venom were aqua-punctured every 2days for 3times to the loci corresponding Choksamni(ST36) in MTX premedicated mice everyday for 3times, and thereafter number of WBC, T-cell, B-cell, lymphocyte proliferation, and splenic natural killer cell activity(NKCA) were also measured.
Bee venom aqua-acupuncture groups divided into 3 groups. BVA I was group treated with dilluted bee venom from bees in Korea, BVAⅡ was group treated with dilluted bee venom from 0.1% of bee venom manufactured by Monmouth Pain Institute Inc. in U.S.A., BVAⅢ was group treated with dilluted bee venom from 0.05% of bee venom manufactured by An Tu Pharmaceutical Factory of Jilin Province in China.
The results were summarized as follows :
1. Number of WBC was increased as compared with the control group in all the bee venom aqua-acupuncture groups. But there was no significance between bee venom aqua-acupuncture groups(BVAⅠ, BVAⅡ, BVAⅢ).
2. Quantitation of T-cell was increased with the statistical significance in all the bee venom aqua-acupuncture groups as compared with the control group. But there was no significance between bee venom aqua-acupuncture groups(BVAⅠ, BVAⅡ, BVAⅢ).
3. Quantitation of B-cell was increased with the statistical significance in all the bee venom aqua-acupuncture groups as compared with the control group. But there was no significance between bee venom aqua-acupuncture groups(BVAⅠ, BVAⅡ, BVAⅢ).
4. Lymphocyte proliferation was increased with the statistical significance in all the bee venom aqua-acupuncture groups as compared with the control group. But there was no significance between bee venom aqua-acupuncture groups(BVAⅠ, BVAⅡ, BVAⅢ).
5. Natural killer cell activity was not increased as compared with the control group in all the bee venom aqua-acupuncture groups. And there was no significance between bee venom aqua-acupuncture groups(BVAⅠ, BVAⅡ, BVAⅢ).
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