The effects of Jowiseungchungtang on the enhancement of learning and memory of AD model rats were studied with Morris water maze and radial arm maze. Sample group was electrolytically lesioned on nbM, and then daily treated with the medicine for two months. Control group with nbM lesion, and sham group with the sham operation were treated the vehicle for same duration. The following results were observed.
1. As the learning trials of Morris water maze processed repeatedly, sham group achieved 200.21±35.28 seconds in 1st trial, 153.14±62.66 seconds in 2nd, 109.93±42.57 seconds in 3rd, 79.29±50.07 seconds in 4th, and 48.07±29.39 seconds in 5th. The control group achieved 224.85±26.94 in 1st trial, 191.77±67.97 seconds in 2nd, 178.46±65.06 seconds in 3rd, 137.46±76.32 seconds in 4th, and 108.92±90.12 seconds in 5th. The sample group achieved 202.00±33.53 seconds in 1st trial, 196.17±40.65 seconds in 2nd, 172.50±56.30 seconds in 3rd, 88.75±47.70 seconds in 4th, and 84.17±59.73 seconds in 5th. Therefore, these data shows that all three groups were improved in learning capacity as trials were repeated, but the sample group showed more prominent improvement in learning compared with the control group(p<0.05).
2. In memory retention test of Morris water maze that counts the staying time in the target area, sham group stayed for 15.36±5.39 seconds, the control group stayed for 5.54±5.64 seconds, and the sample group stayed for 10.08±5.45 seconds. The analysis of the memory retention data shows that the sample group marked more significant improvement statistically in memory retention compared with the control group(p<0.05).
3. In the learning of radial arm maze, the number and rate of animals that arrive the learning criteria amounted 9 out of 14, 64.29% in sham group, none out of 13.0% in the control group, and 3 out of 12, 25.00% in the sample group. So, the sample group shows better learning capacity significantly compared with the control group(p<0.05).
With the experimental results above, Jowiseungchungtang can be supposed to have the improving effects on the learning and memory of AD rats induced by eletronical injury of nbM.
|