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KMID : 0352319990220010039
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Journal of KyungHee Oriental Medicine College
1999 Volume.22 No. 1 p.39 ~ p.65
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Study on the Effects of Curcuma(鬱金) on Angiogenic Inhibition Mechanism
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Sung Hee-Keun
Ahn Kyoo-Seok
Choi Seung-Hoon
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Abstract
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This experimental study was carried out to evaluate the effects of Curcuma on angiogenic inhibition mechanism. In order to investigate the effects of Curcuma on angiogenic inhibition mechanism evaluate cell survival rate by MTT assay, cell adhesive inhibition effect, DNA fragmantaion analysis. Nuclear Condensation assay FACScan analysis, angiogenesis inhibition assay. Immunocytochemistry analysis, RT-PCR for mRNA expression. Western Blot analysis.
The results were summarized as follows :
1. The IC50 was 10㎍/ml to ECV 304 cell.
2. The cell adhesive inhibition ability was strong from 5㎍/ml.
3. The G0/G₁arrest peak was existed on ECV304 cell-line.
4. The cells on Collagen plate were inhibition of proliferation and inducement of apoptosis by Cur water extract.
5. Lumen formation was inhibited by Cur water extract.
6. LFA-1 and ELAM-1’s expression were inhibited by Cur water extract, they are
commenly participation on inflammation and tumor regeneration.
7. The expression of MMP-9 and uPA were inhibited by Cur water extract.
8. The expression of integrin αvβ₃ was inhibited by Cur water extract.
9. The expression of intracellular molecule were successively inhibited by Cur water extract therefore the proliferation of ECV304 cell line was stopped and apoptosis was induced.
According to the results Curcuma has been shown to be a key antagonist of intergrin αvβ₃and which seems to be apoptosis by P53 through Flow cytometry. This report demonstrated that expression of MMP-9 and uPA blocked to under angiogenesis model. Thus, we suggested that Curcuma water block angiogenesis by inducing unscheduled apoptosis in ECV304 and ECVPAR cell lines and another orient herbal medicine that treat Qi-stagnation and Blood-stasis type also have angiogenic inhibition effects.
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KEYWORD
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