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KMID : 0352319990220010155
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Journal of KyungHee Oriental Medicine College
1999 Volume.22 No. 1 p.155 ~ p.169
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Effects of the Chungshimbohyeol-tang on the Gene Expression in Atherosclerotic Lesions
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Song Mi-Deok
Lee Kyung-Sub
Moon Sang-Kwan
Ko Chang-Nam
Kim Young-Suk
Cho Ki-Ho
Bae Hyung-Sup
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Abstract
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The effects of the Chungshimbohyeol-tang on the expression of the MCP-1 and tissue factor genes which are important in the development of atherosclerotic lesions were investigated in the THP-1 human monocyte cell line using reverse transcription- polymerase chain reaction (RT-PCR). Treatment of the THP-1 cells with LPS and the Chungshimbohyeol-tang for 18 hr decreased the expressions of both MCP-1 and tissue factor mRNA in a dose dependent manner. Since it has been reported that NF-kB and AP-1, nuclear transcription factors, are involved in the transcriptional regulation of the MCP-1 and tissue factor gene, effects of the Chungshimbohyeol-tang on the activities of NF-kB and AP-1 were examined using a transient transfection assay and electrophoretic mobility shift assay(EMSA). When the THP-1 cells were transiently transfected with the plasmid containing 3 copies of the NF-kB binding site and the chloramphenicol acetyltransferase (CAT) gene, CAT activity was inhibited by the Chungshimbohyeol-tang. Treatment of the Chungshimbohyeol-tang inhibited protein/DNA binding of NF-kB to its cognate site as measured by EMSA. The Chungshimbohyeol-tang also inhibited AP-1 activation and its binding to DNA. Nuclear concentrations of NF-kB components and AP-1 proteins were also decreased by the treatment of the Chungshimbohyeol-tang. These results suggest that the Chungshimbohyeol-tang could prevent atherosclerosis through the down regulation of the MCP-1 and tissue factor gene expression which might be mediated by the inhibition of NF-kB and AP-1 activities and their binding to DNA.
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KEYWORD
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