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KMID : 0354619920040010199
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Journal Dankook Dental Research Institute
1992 Volume.4 No. 1 p.199 ~ p.212
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Effects of Lipopolysaccharide on the Activity of Osteoclast
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Abstract
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To study the effect of lipopolysaccharide (LPS) on the activity of osteoclast, five bone cell populations were isolated by sequential onzyme digestion of fetal rat calvaria and effect of LPS on the acid and alkaline phosphatase activity was
studied. And
also, effect of LPS on the 45Ca release from fetal rat ulnae and radii, and effects of carbonic anhydrase inhibitors on the LPS-induced bone resorption in organ culture were studied.
Calvaria from rat fetus at 19th day of gestation, were sequentially digested y enzyme solution (collagenase, trypsin and EDTA) for 10, 10, 10, 20 and 20 min. (population I-V). Effect of LPS on the acid and alkaline phosphatase activity of each
bone
cell
population was determined after 24 hr-incubation with 1㎍/ml or 10㎍/ml PLS. Ulnae and radii were removed form 19-day old fetal rats, prelabelled by subcutaneous injection of 200μCi 45CaCl2 into their mother on the 17 th day of gestation.
Afacter
2
hours, media was changed with media containing LPS (1㎍/ml or 10㎍/ml) or LPS + carbonic anhydrase inhibitors (10mM sulfanilamide or 1 mM dichlorphenamide) and cultured for 72 hours. Radioactivities of 45Ca released into media were determined
after
24,
48 and 72 hours. Effects of LPS and carbonic anhydrase inhibitors were observed by the ratio of %-release of 45Ca between paired control and experimental group.
@ES The observed results were as follows :
@EN 1. Basal activities of acid phosphatase were higher in early release cell populations than late populations and basal level of alkaline phosphatase was reversed.
2. LPS (1㎍/ml and 10㎍/ml) increased the acid phosphatase activity in pupulations I and II significantly.
3. LPS (1㎍ml and 10㎍/ml0 decreased the alkaline phosphatase activity of all bone cell populations and significantly inhibited the enzyme activity in populations II, III, IV and V.
4. LPS supplemented in media for 72 hours increased the 45Ca release significantly after 48 and 72 hours of culture.
5. LPS0induced 45Ca release was sinhibited significantly by 10mM sulfanilamide after 72 hours of culture.
6. LPS-induced 45Ca release was inhibited significantly by 1 mM dichlorphenamide after 48 and 72 hours of culture.
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