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KMID : 0354619920040010213
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Journal Dankook Dental Research Institute
1992 Volume.4 No. 1 p.213 ~ p.226
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Effects of the Bacterial Endotoxin on the Proliferation and Prostaglandin Synthesis of the Periodontal Tissue Cells in Culture
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Abstract
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The purpose of this study was to examine the effects of the bacterial endotoxin (lipopolysaccharide, LPS) obtained from Porphyromonas (Bacteroides) gingivalis on the proliferation and prostaglandin E2 (PGE2) synthesis of cultural gingival
fibroblasts
(GFB) and periodontal ligament cells (PDL).
Gingival fibroblasts and periodontal ligament cells were obtained from the cultured periodontal tissues of the sound maxillary premolar which was extracted for the orthodontic purpose.
The cellular proliferations were examined through the quantitive analysis of [3H]-thymidine and the amounts of synthetic PGE2 were checked by a radioimmuno assay kit (RIA) according to the specific concentrations of LPS.
@ES The results were as follows :
@EN 1. The cellular proliferations of GFB and PDL were significantly reduced to about 72% at the concentration of 10.0 ㎍/ml of LPS of PDL.
2. The releasing amounts of synthetic PGE2 were significantly increased at 1.0 and 10.0 ㎍/ml of LPS in GFB, and 0.01, 0.1, 1.0 and 10.0㎍/ml of LPS in PDL.
3. The releasing amounts of synthetic PGE2 in PDL were substantially more than in GFB at each concentration of LPS.
4. The cellular proliferations were significantly inhibited by LPS in the early stage, and the amounts of synthetic PGE2 had the tendency to increase during the time period.
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