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KMID : 0354619920040010227
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Journal Dankook Dental Research Institute
1992 Volume.4 No. 1 p.227 ~ p.244
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Electron Microscopic Study on Human Inflamed Junctional Eplthelium
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Abstract
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The purpose of this study was to investigate the ultrastructural features of human inflammed junctional epithelium. The tissue specimens were taken from a patient with severe periodontitis. After extraction of tooth with not detached junctional
epithelium, the tissue were fixed with 2.5% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.4) for 5 hours and decalcified with 0.1M EDTA solution for 12 weeks. The tissue was postfixed with 1% osmium tetroxide in 0.1M cacodylate buffer and
dehydrated
and embedded in Epon 812. For light microscopic observation, the specimen was cut into the 1㎛ semithin sections and stained with toluidine blue. For electron microscopic observation, the specimen was cut into the 80-90 nm and stained with
uranylacetate
and lead citrate and examined with JEOL 1200 electron microscope.
@ES The results were as follows :
@EN 1. At light microscope, the outer surfaces of inflamed junctional epithelium wee attached to cementum, it's inner surface were projected digitally to connective tissue.
2. Electron microscope revieled that the basal cell of inflammed junctional epithelium showed numerous desmosomes and basal lamina, but basal lamina had lost it's continuity.
3. Intercellular spaces of junctional epithelium of periodontal pocket were wide than that of normal junctional epithelium and reduced number of desmosomes.
4. many vacuoles and loss of continuity of basal lamina were observed at the attached junctional epithelium to the tooth surface.
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KEYWORD
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